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DLL4 Antibody

CAT: 0013-GTR18184452Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18184452Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
DELTA-4 antibody
Product Name Alternative
Rabbit anti-DELTA4 antibody, DELTA-4, delta 4 precursor, Delta-like protein 4, DLL4, Drosophila Delta homolog 4, notch ligand DLL4 precursor, DELTA 4
UniProt
Q9NR61
Reactivity
Human, Mouse
Immunogen
Anti-DELTA-4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human DELTA-4.
Target
DLL4
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0mg/ml
Dilution
ELISA: 1:5,000 - 1:25,000, IHC: 10 μg/mL to 20 μg/mL, WB: 1:500 - 1:2,500
Purity
Anti-DELTA-4 affinity purified antibody is directed against human DELTA-4. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. However, cross reactivity is expected with mouse, rat and chimpanzee DELTA-4 based on a 91% homology to the core 12 amino acid sequence within the 14 amino acid immunogen sequence. Cross reactivity with DELTA-4 homologues from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-DELTA-4 has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 74 kDa in size corresponding to DELTA-4 by western blotting in the appropriate cell lysate or extract. For best results use Goat Serum as blocking agent in western blotting.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
9506545
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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