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ROBO1 Antibody

CAT: 0013-GTR18184450Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18184450Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ROBO-1 antibody
Product Name Alternative
Rabbit anti-ROBO1 antibody, ROBO 1, ROBO-1, hROBO-1, Roundabout homolog 1, Deleted in U twenty twenty, DUTT1, DUTT-1
UniProt
Q2M1J3
Reactivity
Human, Mouse
Immunogen
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an C-Terminal region near amino acids 1625-1650 of Human ROBO-1.
Target
ROBO1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Concentration
1.0 mg/mL
Dilution
ELISA: 1:30,000 - 1:160,000, IHC: 2 μg/ml to 10 μg/ml, WB: 1:500 - 1:3,000
Purity
This affinity purified antibody is directed against human ROBO-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat and dog sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of ROBO-1. Cross reactivity with ROBO-1 homologues from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA, western blot, and immunohistochemistry. It may be suitable for immunofluorescence and IP. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~181 kDa in size corresponding to ROBO-1 by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
AAI12337.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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