IKBKE Antibody

CAT: 0013-GTR18184409Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18184409Size:100 µg

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Description

IKK E (phospho-T501) antibody

Product Name Alternative

Rabbit anti-IKK epsilon pT501 antibody, rabbit anti-IKKE pT501 antibody, I kappa B kinase epsilon antibody, IKK-epsilon, IKK-E, Inducible I kappa-B kinase, IkBKE antibody, IKBKE protein antibody, Inhibitor of nuclear factor kappa B kinase subunit epsilon antibody, IKKE, IKKI

UniProt

Q14164

Reactivity

Human

Immunogen

IKKe phospho peptide corresponding to a region of the human protein surrounding pT501 conjugated to KLH.

Target

IKBKE

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Concentration

1 mg/mL

Dilution

ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000

Purity

Anti-IKKε pT501 antibody was affinity purified from monospecific antiserum by immunoaffinity purification against the phosphopeptide and cross adsorption against the non-phosphorylated form of the peptide followed by non-adsorption against a non-specific peptide backbone to further reduce non-specific reactivity. This phospho specific polyclonal antibody is specific for phosphorylated pT501 human IKKe. Reactivity with non-phosphorylated IKKe is minimal. Cross reactivity with pT501 phosphorylated IKKe from mouse, rat or other species has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

IKKε pT501 antibody is tested in ELISA, western blotting, and although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. An 85 kDa band corresponding to human IKKe is detected. HeLa cells or TNF inducible KBM-5 cells can be used as a positive control. Researchers should determine optimal titers for other applications.

Tested Applications

ELISA, WB

NCBI Accession Number

Q14164.1

Host or Source

Rabbit

Preservative

Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · Q14164

Inhibitor of nuclear factor kappa-B kinase subunit epsilon

IKKE_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: Q14164
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: IKBKE
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: I-kappa-B kinase epsilon, IKK-E, IKK-epsilon, IkBKE
EC number: 2.7.11.1, 2.7.11.10
Processing: —
Secondary accessions: D3DT78, Q3B754, Q3KR43, Q5JTS6

Protein keywords

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingNucleotide-binding

Cellular component

CytoplasmNucleus

Biological process

DNA damageHost-virus interaction

PTM

Isopeptide bondPhosphoproteinUbl conjugation

Molecular function

KinaseSerine/threonine-protein kinaseTransferase

Technical term

Proteomics identificationReference proteome

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