COL1A1/A2 Antibody

CAT: 0013-GTR18184376Size: 500 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18184376Size:500 µg

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Description

Collagen I antibody

Product Name Alternative

Rabbit anti-collagen type I antibody, Collagen Of Skin Tendon And Bone, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen alpha-1 (I) chain, Alpha-1 type I collagen, type 1 procollagen alpha 1, Collagen type I alpha 2 antibody

UniProt

P02452

Reactivity

Bovine, Human, Mouse, Porcine, Rat

Immunogen

Collagen Type I from human and bovine placenta

Target

COL1A1/A2

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Musculoskeletal & Connective Tissue Research

Concentration

1.06 mg/mL

Dilution

ELISA: 1:5,000 - 1:50,000, FLISA: 1:100, IHC: 1:50 - 1:200, IP: 1:100, WB: 1:1,000 - 1:10,000

Purity

COLLAGEN I Antibody has been prepared by immunoaffinity chromatography using immobilized antigens. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Collagen Type I has been tested by dot blot and IHC and are useful for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, Immunofluorescence, FLOW, and western blotting for highly sensitive qualitative analysis.

Tested Applications

DOT, ELISA, FLISA, IHC, IP, WB

NCBI Accession Number

NP_000079.2

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · P02452

Collagen alpha-1(I) chain

CO1A1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P02452
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: COL1A1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: O76045, P78441, Q13896, Q13902, Q13903, Q14037, Q14992, Q15176, Q15201, Q16050, Q59F64, Q7KZ30, Q7KZ34, Q8IVI5, Q8N473, Q9UML6, Q9UMM7

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Ligand

CalciumMetal-binding

Coding sequence diversity

Chromosomal rearrangement

Domain

CollagenRepeatSignal

Disease

Disease variantDwarfismEhlers-Danlos syndromeOsteogenesis imperfectaOsteoporosis

PTM

Disulfide bondGlycoproteinHydroxylationPhosphoproteinPyrrolidone carboxylic acid

Cellular component

Extracellular matrixSecreted

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