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Tnfsf11 Antibody

CAT: 0013-GTR18184287Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184287Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
RANK-L antibody
Product Name Alternative
Rabbit anti-RANK-L Antibody, Receptor activator of nuclear factor kappa B, TNF-related activation-induced cytokine, TRANCE, Tumor necrosis factor ligand superfamily member 11, TNF11, TNFRSF11, Osteoprotegerin ligand, OPG, Osteoclast differentiation factor, ODF, CD254
UniProt
O35235
Reactivity
Mouse
Immunogen
This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated mouse RANKL protein (aa 143-316) produced in E.coli.
Target
Tnfsf11
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Concentration
1.0 mg/mL
Dilution
ELISA: 1:10,000, WB: 1:1000
Purity
Anti-RANK L antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse RANKL protein. A BLAST analysis was used to suggest cross-reactivity with RANKL from mouse sources based on 100% homology with the immunizing sequence. Based on 97% homology, cross-reactivity with rat, 91% with Chinese hamster, 90% with Thirteen-lined ground squirrel; other sources has not been determined.
Form
Lyophilized
Storage Conditions
Store antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This purified antibody has been tested in western blotting and suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19-20 kDa in size corresponding to the mature mouse RANKL protein by western blotting in appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_035743.2
Host or Source
Rabbit
Preservative
Preservative: None. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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