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Csf2 Antibody

CAT: 0013-GTR18184278Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184278Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
GM-CSF antibody
Product Name Alternative
Rabbit anti-GM-CSF antibody, rabbit anti-Granulocyte-Macrophage colony-stimulating factor antibody, CSF, sargramostim, molgramostin
UniProt
P01587
Reactivity
Mouse
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse GM-CSF protein.
Target
Csf2
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/mL
Dilution
ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000
Purity
This purified antibody has been purified by a multi-step process which includes delipidation, salt fractionation, and heat treatment to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse GM-CSF in cell supernatants and certain body fluids. This antibody is useful for neutralization of mouse GM-CSF in bioassays. For neutralization, it is recommended to incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This purified antibody has been tested in western blot and is suitable for use in neutralizations and ELISA. Reactivity is also expected in radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/μl by the LAL method. By western blot a band approximately 14.3 kDa in size corresponding to mouse GM-CSF protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_034099.2
Host or Source
Rabbit
Preservative
Preservative: None. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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