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Il6 Antibody

CAT: 0013-GTR18184277Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184277Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
IL6 antibody
Product Name Alternative
Rabbit anti-IL-6 antibody, rabbit anti-Interleukin-6 antibody, Interleukin6 cytokine, IL6, B-cell stimulatory factor 2, BSF-2, Interferon beta-2, IFN-beta-2, Hybridoma growth factor, CTL differentiation factor, CDF, Interleukin HP-1
UniProt
P08505
Reactivity
Mouse
Immunogen
Anti-IL-6 is an IgG fraction antibody prepared from rabbit antiserum after repeated immunizations with recombinant mouse IL-6 protein produced in E.coli.
Target
Il6
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Concentration
1.0 mg/mL
Dilution
ELISA: 1:10,000, WB: 1:1000
Purity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse IL-6 protein. A BLAST analysis was used to suggest cross-reactivity with IL-6 from mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-6 from other sources has not been determined.
Form
Lyophilized
Storage Conditions
Store anti-IL-6 at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This purified antibody has been tested for use in Immunohistochemistry and western blotting and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21.7 kDa in size corresponding to the mature 187 amino acid mouse IL-6 protein by western blotting in appropriate cell lysate or extract.
Tested Applications
ELISA, FC, IHC, WB
NCBI Accession Number
NP_112445.1
Host or Source
Rabbit
Preservative
Preservative: None. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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