Enzyme Donor Stabilization Buffer

• Description:

  Enzyme Donor Stabilization Buffer (Beta-galactosidase). _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_

  Catalog #	B2010001
  Size	50 mL
  Storage	2° - 8°C.
  Buffer	Buffered solution at pH 7.4 with proprietary stabilizers and preservatives specially formulated for the enzyme donor.
  Application	Alpha complementation kit, cloned enzyme donor immunoassays, ELISA.
  Keywords	Beta-galactosidase Alpha Peptide, Beta-galactosidase Enzyme Donor, Beta-galactosidase Omega Domain, Enzyme Acceptor, Alpha Complementation, LacZ.
  Related products	Beta-galactosidase Enzyme Donor (Alpha Peptide), Stabilization Buffer for Enzyme Acceptor, Beta-galactosidase Enzyme Acceptor (Omega Domain), Beta-galactosidase Enzyme Acceptor Stabilization Buffer, Beta-galactosidase Chromogenic Substrate.

  _x000D_  _x000D_

  About Beta-galactosidase Alpha Complementation

  _x000D_

  Beta-galactosidase Alpha-Complementation is a biochemical phenomenon first documented by Agnes Ullmann, while working in the lab of François Jacob and Jacques Monod. By means of molecular cloning, the native E. coli β-galactosidase enzyme can be split in two inactive fragments of different sizes. The smaller fragment, known as the alpha-peptide or enzyme donor, is about 100 amino residues in length and is inactive on its own (incapable of hydrolyzing a β-galactosidase substrate). The larger fragment, known as the omega fragment or enzyme acceptor, is about 900 amino residues in length and is also inactive on its own. Upon mixing the enzyme donor with the enzyme acceptor, the β-galactosidase enzyme is reconstituted and is now capable of hydrolyzing colorimetric substrates such as ONPG.

  _x000D_

  Both enzyme donor and enzyme acceptor can be cloned and expressed in special E. coli strains to yield highly pure, zero-background enzyme fragments (i.e. an enzyme donor and enzyme acceptor without measurable catalytic activities, when assayed individually). Interestingly, it was discovered that various analytes can be conjugated to the enzyme donor moiety and the enzyme donor-enzyme acceptor association modulated by an analyte-binding molecule (such as an antibody). As a result, an alpha-complementation-based assay can be developed.

  _x000D_ Download Alpha Complementation Product Line Brochure._x000D_

  References

  _x000D_

  _x000D_
  • Kras, E. (2019). Beta-galactosidase: properties, structure and functions. New York: Nova Science Publishers.
  _x000D_
  • Arndt, T. (2017). Cloned Enzyme Donor Immunoassay. Lexikon Der Medizinischen Laboratoriumsdiagnostik, 1€“2.
  _x000D_
  • Jeon, S. I., Yang, X., and Andrade, J. D. (2004). Modeling of homogeneous cloned enzyme donor immunoassay. Analytical Biochemistry, 333(1), 136€“147.
  _x000D_
  • Tachi, T., Kaji, N., Tokeshi, M., and Baba, Y. (2009). Microchip-based Homogeneous Immunoassay Using a Cloned Enzyme Donor. Analytical Sciences, 25(2), 149€“151.
  _x000D_
  • Khanna, P. L., and Worthy, T. E. (1993). CEDIA: A Recombinant-Based Homogeneous Enzyme Immunoassay.
  _x000D_

  _x000D_

  Related products

  _x000D_ https://moleculardepot.com/product/beta-galactosidase-enzyme-donor/_x000D_ _x000D_ https://moleculardepot.com/product/beta-galactosidase-enzyme-acceptor/_x000D_ _x000D_ https://moleculardepot.com/product/enzyme-acceptor-stabilization-buffer/_x000D_ _x000D_ https://moleculardepot.com/product/beta-galactosidase-alpha-complementation-kit/_x000D_ _x000D_ https://moleculardepot.com/product/cprg-chlorophenol-red-β-d-galactopyranoside/

• Short Description:

  Catalog #: B2010001 (50 mL)_x000D_ _x000D_ _x000D_ Beta-galactosidase Enzyme Donor Stabilization Buffer (50 mL) consists of a buffer specifically optimized to stabilize the beta-galactosidase fragment known as enzyme donor (Alpha fragment). The buffer can extend the shelf life of the enzyme donor to up to 12 months at 2-8C. Without this buffer, the enzyme donor is highly instable and loses activity at a high rate. Custom bulk orders of this product are available upon request._x000D_ _x000D_ Live Enquiry about this product via Text/SMS: 1-858-900-3210 (8 am €“ 8 pm PST)

• Weight:

  2.6

• Length:

  3.3

• Width:

  1.2

• Height :

  1.2