Products for Research Use Only

IL4 Antibody (Biotin)

CAT: 0013-GTR18184255Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184255Size:100 µg
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Description
IL4 antibody (Biotin)
Product Name Alternative
Rabbit anti-IL-4 biotin conjugated antibody, rabbit anti-interleukin-4 biotin conjugated antibody, BCGF-1, BCGF1, Binetrakin, BSF 1, BSF1, HCGF, IA Inducing Factor, IGG1 induction factor, IL 4, Il4e12, Interleukin 4, Interleukin 4, isoform 1, Interleukin4, Lymphocyte stimulatory factor 1, MCGF2, MFF, Pitrakinra, TCGF2
UniProt
P05112
Reactivity
Human
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-4 protein.
Target
IL4
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Immunology & Inflammation
Concentration
0.80 mg/ml
Dilution
ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
Purity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-4 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This purified antibody has been tested in western blotting and suitable for ELISA. By western blot a band approximately 15 kDa in size corresponding to human IL-4 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_000580.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P05112

Interleukin-4

IL4_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P05112
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IL4
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IL-4
EC number
—
Processing
Precursor
Secondary accessions
Q14630, Q6NZ77
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

B-cell activation

Molecular function

CytokineGrowth factor

PTM

Disulfide bondGlycoprotein

Cellular component

Secreted

Domain

Signal