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IL3 Antibody (HRP)

CAT: 0013-GTR18184240Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184240Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
IL-3 antibody (Peroxidase)
Product Name Alternative
Rabbit anti-IL-3 peroxidase antibody, rabbit anti-Interleukin 3 peroxidase antibody, IL3, interleukin-3, Burst promoting activity, Colony stimulating factor, Eosinophil colony stimulating factor, Hematopoietic growth factor, IL-3, Hematopoietic growth factor, Mast cell growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor
UniProt
P08700
Reactivity
Human
Immunogen
Anti-Human IL-3 (RABBIT) Antibody Peroxidase Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-3 protein.
Target
IL3
Clonality
Polyclonal
Conjugation
HRP
Field of Research
Immunology & Inflammation
Concentration
1.0 mg/mL
Dilution
ELISA: 1:10,000 - 1:50,000, IHC: 1:500-1:2,500, WB: 1:1,000-1:5,000
Purity
Anti-Human IL-3 (RABBIT) Antibody Peroxidase Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-3 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Form
Lyophilized
Storage Conditions
Store Anti-Human IL-3 (RABBIT) Antibody Peroxidase Conjugated at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This IL-3 antibody has been tested in western blotting and suitable for ELISA. By western blot a band approximately 15 kDa in size corresponding to human IL-3 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_000579
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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