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IL6 Antibody (HRP)

CAT: 0013-GTR18184232Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184232Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
IL6 antibody (Peroxidase)
Product Name Alternative
Rabbit Anti-IL-6 peroxidase conjugated antibody, rabbit anti-interleukin-6 peroxidase conjugated antibody, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF
UniProt
P05231
Reactivity
Human
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-6 produced in E.coli.
Target
IL6
Clonality
Polyclonal
Conjugation
HRP
Field of Research
Immunology & Inflammation
Concentration
1.1 mg/ml
Dilution
ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,500, WB: 1:1,000-1:5,000
Purity
This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human Il-6 in cell supernatants and certain body fluids. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. A control of similarly diluted normal rabbit IgG is recommended. This product has minimal reactivity with mouse IL-6.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This purified antibody has been tested in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/μl by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_000591.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P05231

Interleukin-6

IL6_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P05231
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IL6
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IL-6
EC number
—
Processing
Precursor
Secondary accessions
Q9UCU2, Q9UCU3, Q9UCU4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Acute phase

Molecular function

CytokineGrowth factor

PTM

Disulfide bondGlycoproteinPhosphoprotein

Cellular component

Secreted

Domain

Signal

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