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BDNF Antibody

CAT: 0013-GTR18184225Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184225Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
BDNF antibody
Product Name Alternative
Rabbit anti-BDNF, rabbit anti-brain derived neurotropic factor, Brain-derived neurotrophic factor, Abrineurin cytokine
UniProt
P23560
Reactivity
Human
Immunogen
This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated human BDNF protein produced in E.coli.
Target
BDNF
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Concentration
1.0 mg/mL
Dilution
ELISA: 1:10,000, WB: 1:1000
Purity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human BDNF protein. A BLAST analysis was used to suggest cross-reactivity with BDNF from human sources based on 100% homology with the immunizing sequence. Based on high to 100% homology, there is a chance of cross-reactivity to BDNF from a wide variety of animals. Cross-reactivity with BDNF from other sources has not been determined.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This purified antibody has been tested in western blotting and suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 27 kDa in size corresponding to the mature human BDNF protein by western blotting in appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
CAA62632.1
Host or Source
Rabbit
Preservative
Preservative: None. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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