Products for Research Use Only
RegisterLogin

IL6 Antibody

CAT: 0013-GTR18184203Size: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18184203Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
IL6 antibody
Product Name Alternative
Rabbit Anti-IL-6 antibody, rabbit anti-interleukin-6 antibody, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF
UniProt
P05231
Reactivity
Human
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with mature length recombinant human IL-6 produced in E.coli.
Target
IL6
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Concentration
1.0 mg/mL
Dilution
ELISA: 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:300 - 1:1,000
Purity
IL-6 antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts) . In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human of IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity. This product has minimal reactivity with mouse IL-6.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This purified antibody has been tested for use in IHC and western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/μl by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_000591.1
Host or Source
Rabbit
Preservative
Preservative: None. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

Related Products

CatalogName
GTR18225737IL6R/IL6 Receptor Antibody
GTR18221828IL6R/IL6 Receptor Antibody
GTR18221816IL6R/IL6 Receptor Antibody
GTR18224649IL6R/IL6 Receptor Antibody
GTR18579621IL6 Biosimilar Antibody
GTR18579042-01IL6 Biosimilar Antibody
GTR18796163-01IL6 Biosimilar Antibody
GTR18796154-01IL6 Biosimilar Antibody
GTR18578795-01IL6 Biosimilar Antibody
GTR18039516-01IL6 polyclonal antibody

Alternative Products

CatalogName
MBS8529199-01H2B Antibody Antibody
MBS8513599-01CD11b Antibody Antibody
DF8746-01ASAP1 antibody Antibody
MBS850197-01Antibody to IGFBP2 Antibody
E36403ZNF98 Antibody Antibody
GWB-250026Lck antibody Antibody
E8M1007-4HSP60 Antibody Antibody
MBS9403836-01SZT2 Antibody Antibody
LSMab09882anti-Antibody^Polyclonal antibody control antibody
GWB-5C6547MAD1L1 antibody [9B10] Antibody

Popular Products