Products for Research Use Only

IFN gamma Antibody

CAT: 0013-GTR18184175Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184175Size:100 µg
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Description
IFN gamma antibody
Product Name Alternative
Rabbit anti-Interferon gamma, IFNG, rabbit anti-IFN gamma
UniProt
D3GGX1
Reactivity
Gallus
Immunogen
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to 145 amino acids of mature chicken IFN gamma.
Target
IFN gamma
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Infectious Disease & Virology
Concentration
1.0 mg/mL
Dilution
ELISA: 1:20,000, WB: 1:500-1:2,000
Purity
This product was protein A purified from monospecific antiserum by chromatography. This antibody is specific for chicken IFN gamma protein. A BLAST analysis was used to suggest cross-reactivity with IFN gamma from chicken based on 100% homology; ring-necked pheasant, turkey, quail, and junglefowl based on 91-97% homology with the immunizing sequence. Cross-reactivity with IFN gamma from other sources has not been determined.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This protein A purified IFN-gamma antibody has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 16.7 kDa in size corresponding to chicken IFN gamma by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_990480.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P49708

Interferon gamma

IFNG_CHICK · Gallus gallus

View on UniProt ↗
Primary accession
P49708
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IFNG
Protein existence
2: Evidence at transcript level
Organism
Gallus gallus (Chicken)
Taxonomy ID
9031
Alternative names
IFN-gamma
EC number
—
Processing
Precursor
Secondary accessions
D3GGX1, Q53YS2
Protein keywords

Biological process

Antiviral defenseGrowth regulation

Molecular function

Cytokine

PTM

Glycoprotein

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal