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DAO Antibody (Biotin)

CAT: 0013-GTR18184134Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184134Size:100 µg
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Description
D-Amino Acid Oxidase antibody (Biotin)
Product Name Alternative
Sheep anti-D-Amino Acid Oxidase biotin conjugated Antibody, biotin conjugated sheep anti-D-Amino Acid Oxidase Antibody, D-amino-acid oxidase, DAMOX, DAAO, DAO
UniProt
P00371
Reactivity
Porcine
Immunogen
D-Amino Acid Oxidase [Pig Kidney]
Target
DAO
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Metabolism Research
Concentration
1.0 mg/mL
Dilution
ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
Purity
Anti-D-Amino Acid Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified D-Amino Acid Oxidase [Pig Kidney]. Cross reactivities against D-Amino Acid Oxidase from other sources may occur but have not been specifically determined.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-D-Amino Acid Oxidase has been assayed against 1.0 ug of D-Amino Acid Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:16,000 to 1:68,000 of the reconstitution concentration is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent) . Reconstitution Volume: 100 μL
Tested Applications
ELISA, IP, WB
NCBI Accession Number
NP_999231.1
Host or Source
Sheep
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P00371

D-amino-acid oxidase

OXDA_PIG · Sus scrofa

View on UniProt ↗
Primary accession
P00371
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
DAO
Protein existence
1: Evidence at protein level
Organism
Sus scrofa (Pig)
Taxonomy ID
9823
Alternative names
DAAO, DAMOX, DAO
EC number
1.4.3.3
Processing
—
Secondary accessions
A0A287AV04, A0A4X1SRS8
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Cellular component

Cell projectionCytoplasmPeroxisomeSecretedSynapse

Ligand

FADFlavoprotein

Molecular function

Oxidoreductase

PTM

PhosphoproteinS-nitrosylation

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