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GPT Antibody

CAT: 0013-GTR18184118Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18184118Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Alanine Transaminase antibody
Product Name Alternative
Sheep anti-Alanine Transaminase Antibody, AAT1 antibody, Alanine aminotransferase 1 antibody, alanine aminotransferase antibody, ALT 1 antibody, ALT1 antibody, Glutamic alanine transaminase 1 antibody
UniProt
P13191
Reactivity
Porcine
Immunogen
Alanine Transaminase (ALT) [Pig Heart]
Target
GPT
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Concentration
1 mg/mL
Dilution
ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
Purity
Anti-ALANINE TRANSAMINASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Alanine Transaminase [Pig Heart]. Cross reactivity against Alanine Transaminase from other sources is unknown.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Alanine Transaminase antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Alanine Transaminase [Pig Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Tested Applications
ELISA, WB
NCBI Accession Number
113565
Host or Source
Sheep
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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