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GPT Antibody

CAT: 0013-GTR18184117Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184117Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Alanine Transaminase antibody
Product Name Alternative
Sheep anti-Alanine Transaminase Antibody, AAT1 antibody, Alanine aminotransferase 1 antibody, alanine aminotransferase antibody, ALT 1 antibody, ALT1 antibody, Glutamic alanine transaminase 1 antibody
UniProt
P13191
Reactivity
Porcine
Immunogen
Alanine Transaminase (ALT) [Pig Heart]
Target
GPT
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Concentration
1 mg/mL
Dilution
ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
Purity
Anti-ALANINE TRANSAMINASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Alanine Transaminase [Pig Heart]. Cross reactivity against Alanine Transaminase from other sources is unknown.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Alanine Transaminase antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Alanine Transaminase [Pig Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Tested Applications
ELISA, WB
NCBI Accession Number
113565
Host or Source
Sheep
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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