LPO Antibody

CAT: 0013-GTR18184114Size: 25 µLDry Ice: NoHazardous: No

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CAT#:0013-GTR18184114Size:25 µL

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Description

Lactoperoxidase antibody

Product Name Alternative

Sheep anti-Lactoperoxidase Antibody, LPO antibody, Salivary peroxidase antibody, SAPX antibody, SPO antibody

UniProt

P80025

Reactivity

Bovine

Immunogen

Lactoperoxidase [Bovine Milk]

Target

LPO

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Metabolism Research

Concentration

1 mg/mL

Dilution

ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000

Purity

Anti-LACTOPEROXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Lactoperoxidase [Bovine Milk]. Cross reactivity against Lactoperoxidase from other tissues and species may occur but have not been specifically determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Lactoperoxidase antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Lactoperoxidase [Bovine Milk] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Rabbit) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

Tested Applications

ELISA, IP, WB

NCBI Accession Number

P80025.1

Host or Source

Sheep

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · P80025

Lactoperoxidase

PERL_BOVIN · Bos taurus

View on UniProt ↗

Primary accession: P80025
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: LPO
Protein existence: 1: Evidence at protein level
Organism: Bos taurus (Bovine)
Taxonomy ID: 9913
Alternative names: LPO
EC number: 1.11.1.7
Processing: Precursor
Secondary accessions: —

Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Molecular function

AntibioticAntimicrobialOxidoreductasePeroxidase

Ligand

CalciumHemeIronMetal-binding

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinNitrationPhosphoprotein

Cellular component

CytoplasmSecreted

Biological process

Hydrogen peroxide

Domain

Signal

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