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AKT2 Antibody (APC)

CAT: 0013-GTR18184105Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184105Size:50 µg
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Description
AKT2 APC antibody (APC)
Product Name Alternative
Rat anti-Anti-AKT2 Allophycocyanin Conjugation, APC Conjugated rat anti-Anti-AKT2, AKT 2 antibody, AKT-2, PKB antibody, PKB beta antibody, PKBBETA antibody, PRKBB antibody, Protein kinase Akt 2 antibody, Protein kinase B beta antibody, RAC-beta serine/threonine-protein kinase, RAC-PK-beta
UniProt
P31751
Reactivity
Human
Immunogen
Anti-AKT2 Antibody was produced in rat by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.
Target
AKT2
Clonality
Monoclonal
Clone
11F6.B2
Conjugation
APC
Field of Research
Signal Transduction
Concentration
1.0 mg/mL
Dilution
ELISA: User Optimized, FC: User Optimized, IHC: User Optimized, IF: User Optimized, WB: User Optimized
Purity
Anti-AKT2 antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT2 was purified from concentrated tissue culture supernate by Protein G chromatography. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 88% identity to mouse and 90% identity with rat, therefore, cross reactivity is expected.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent) . This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Notes
For research use only.
Applications Notes
Anti-AKT2 APC Antibody is tested for Flow Cytometry. This antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT2 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
Tested Applications
DOT, FC
NCBI Accession Number
NP_001617.1
Host or Source
Rat
Preservative
Preservative: None. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Isotype
IgG2a

UniProtKB · P31751

RAC-beta serine/threonine-protein kinase

AKT2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P31751
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
AKT2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.7.11.1
Processing
—
Secondary accessions
B2RBD8, Q05BV0, Q0VAN0, Q0VAN1, Q68GC0
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationDisulfide bondGlycoproteinPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Biological process

ApoptosisCarbohydrate metabolismGlucose metabolismGlycogen biosynthesisGlycogen metabolismSugar transportTranslation regulationTransport

Ligand

ATP-bindingManganeseMetal-bindingNucleotide-binding

Cellular component

Cell membraneCytoplasmEndosomeMembraneNucleus

Molecular function

Developmental proteinKinaseSerine/threonine-protein kinaseTransferase

Disease

Diabetes mellitusDisease variantProto-oncogene

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