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CES3 Antibody (Biotin)

CAT: 0013-GTR18184067Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18184067Size:25 µL
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Description
Esterase antibody (Biotin)
Product Name Alternative
Rabbit anti-Esterase Antibody biotin Conjugation, biotin conjugated rabbit anti-Esterase Antibody, Liver carboxylesterase, Proline-beta-naphthylamidase, Retinyl ester hydrolase
UniProt
Q29550
Reactivity
Porcine
Immunogen
Esterase [Porcine Liver]
Target
CES3
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Cell Biology
Concentration
1.0 mg/mL
Dilution
ELISA: 1:500 - 1:2,500
Purity
Anti-Esterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Esterase [Porcine Liver]. Cross reactivities against Esterase from other sources may occur but have not been specifically determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Esterase Biotin Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Esterase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product
Tested Applications
ELISA, WB
NCBI Accession Number
343791015
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · Q29550

Liver carboxylesterase

EST1_PIG · Sus scrofa

View on UniProt ↗
Primary accession
Q29550
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
—
Protein existence
1: Evidence at protein level
Organism
Sus scrofa (Pig)
Taxonomy ID
9823
Alternative names
—
EC number
3.1.1.1
Processing
Precursor
Secondary accessions
O97582, P82128
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

PTM

Disulfide bondGlycoproteinPhosphoprotein

Cellular component

Endoplasmic reticulum

Molecular function

HydrolaseSerine esterase

Domain

Signal

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