Elastase Antibody Biotin Conjugated

CAT: 0013-GTR18184065Size: 25 µLDry Ice: NoHazardous: No

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CAT#:0013-GTR18184065Size:25 µL

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Description

Elastase antibody (Biotin)

Product Name Alternative

Rabbit anti-Elastase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Elastase Antibody, Fecal pancreatic elastase 1 antibody, HLE antibody, Medullasin antibody, Pancreatic elastase IIA antibody, PE 1 antibody, PE1 antibody, Protease E antibody

UniProt

P00772

Reactivity

Porcine

Immunogen

Elastase [Porcine Pancreas]

Target

CELA1

Clonality

Polyclonal

Conjugation

Biotin

Concentration

1.0 mg/mL

Dilution

ELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:2,000

Purity

Anti-Elastase (Rabbit) Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Elastase [Porcine Pancreas]. Cross reactivities against Elastase from other sources may occur but have not been specifically determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Elastase Biotin Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Elastase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested

Tested Applications

ELISA, WB

NCBI Accession Number

NP_998988.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · P00772

Chymotrypsin-like elastase family member 1

CELA1_PIG · Sus scrofa

View on UniProt ↗

Primary accession: P00772
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: CELA1
Protein existence: 1: Evidence at protein level
Organism: Sus scrofa (Pig)
Taxonomy ID: 9823
Alternative names: —
EC number: 3.4.21.36
Processing: Precursor
Secondary accessions: Q29625

Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Ligand

CalciumMetal-binding

PTM

Disulfide bondZymogen

Molecular function

HydrolaseProteaseSerine protease

Cellular component

Secreted

Domain

Signal

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