Products for Research Use Only
RegisterLogin

PhoA Antibody (Biotin)

CAT: 0013-GTR18184020Size: 25 µLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18184020Size:25 µL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Alkaline Phosphatase antibody (Biotin)
Product Name Alternative
Rabbit anti-Alkaline Phosphatase Antibody biotin Conjugation, biotin conjugated rabbit anti-Alkaline Phosphatase Antibody, Akp2 antibody, ALP antibody, Nagao isozyme antibody, Placental alkaline phosphatase antibody, Placental like alkaline phosphatase antibody, PLAP 1 antibody, PLAP like antibody, Regan isozyme antibody, Kasahara isozyme antibody
UniProt
P00634
Reactivity
E. coli
Immunogen
Alkaline Phosphatase [E.coli]
Target
PhoA
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Signal Transduction
Concentration
1.0 mg/mL
Dilution
ELISA: 1:4,000 - 1:20,000, WB: 1:500 - 1:2,000
Purity
Anti-Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [E.coli]. Cross reactivities against Alkaline Phosphatase from other sources may occur but have not been specifically determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Alkaline Phosphatase Biotin Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Alkaline Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product
Tested Applications
ELISA, WB
NCBI Accession Number
NP_414917.2
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P00634

Alkaline phosphatase

PPB_ECOLI · Escherichia coli (strain K12)

View on UniProt ↗
Primary accession
P00634
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
phoA
Protein existence
1: Evidence at protein level
Organism
Escherichia coli (strain K12)
Taxonomy ID
83333
Alternative names
APase
EC number
3.1.3.1
Processing
Precursor
Secondary accessions
P77801, P78051, Q2MC42, Q47041
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

PTM

Disulfide bondPhosphoprotein

Molecular function

Hydrolase

Ligand

MagnesiumMetal-bindingZinc

Cellular component

Periplasm

Domain

Signal

Related Products

CatalogName
GTR17987629PhoA Antibody
GTR17987628PhoA Antibody
GTR18183863PhoA Antibody
GTR17987425PhoA Antibody
GTR18183862PhoA Antibody
GTR18187431PhoA Antibody
GTR18183942PhoA Antibody (HRP)
GTR18183943PhoA Antibody (HRP)
GTR18680436-01E. coli phoA Rabbit Polyclonal Antibody
GTR19071250-01Recombinant E. coli phoA Protein, N-His

Alternative Products

CatalogName
AP21367BT-NphoA Rabbit Polyclonal Antibody
E55A04626E. coli phoA Polyclonal Antibody
ARO-A19242-01Anti-E. coli phoA Polyclonal Antibody
WP20076-01U-Blot® E. coli phoA Polyclonal Antibody
MBS7137919Rabbit anti-Escherichia coli (strain K12) phoA Polyclonal Antibody
MBS7147901Rabbit anti-Bacillus subtilis (strain 168) phoA Polyclonal Antibody
MBS7137920Rabbit anti-Escherichia fergusonii (strain 35469/DSM 13698/CDC 0568-73) phoA Polyclonal Antibody
STJ501938Anti-Noelin Antibody BIOTIN Antibody BIOTIN
V5320SAF-100UGBiotin Antibody
HY-P703429PhoA, E.coli

Popular Products