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PhoA Antibody (Biotin)

CAT: 0013-GTR18184019Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184019Size:100 µg
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Description
Alkaline Phosphatase antibody (Biotin)
Product Name Alternative
Rabbit anti-Alkaline Phosphatase Antibody biotin Conjugation, biotin conjugated rabbit anti-Alkaline Phosphatase Antibody, Akp2 antibody, ALP antibody, Nagao isozyme antibody, Placental alkaline phosphatase antibody, Placental like alkaline phosphatase antibody, PLAP 1 antibody, PLAP like antibody, Regan isozyme antibody, Kasahara isozyme antibody
UniProt
P00634
Reactivity
E. coli
Immunogen
Alkaline Phosphatase [E.coli]
Target
PhoA
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Signal Transduction
Concentration
1.0 mg/mL
Dilution
ELISA: 1:4,000 - 1:20,000, WB: 1:500 - 1:2,000
Purity
Anti-Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [E.coli]. Cross reactivities against Alkaline Phosphatase from other sources may occur but have not been specifically determined.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Alkaline Phosphatase Biotin Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Alkaline Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent) . Reconstitution Volume: 100 μL
Tested Applications
ELISA, WB
NCBI Accession Number
NP_414917.2
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P00634

Alkaline phosphatase

PPB_ECOLI · Escherichia coli (strain K12)

View on UniProt ↗
Primary accession
P00634
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
phoA
Protein existence
1: Evidence at protein level
Organism
Escherichia coli (strain K12)
Taxonomy ID
83333
Alternative names
APase
EC number
3.1.3.1
Processing
Precursor
Secondary accessions
P77801, P78051, Q2MC42, Q47041
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

PTM

Disulfide bondPhosphoprotein

Molecular function

Hydrolase

Ligand

MagnesiumMetal-bindingZinc

Cellular component

Periplasm

Domain

Signal

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