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CA2 Antibody (HRP)

CAT: 0013-GTR18183971Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18183971Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Carbonic Anhydrase II antibody (Peroxidase)
Product Name Alternative
Rabbit anti-Carbonic Anhydrase II Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Carbonic Anhydrase II Antibody, Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody, CAC, CA-II
UniProt
P00921
Reactivity
Bovine
Immunogen
Carbonic Anhydrase II [Bovine Erythrocytes]
Target
CA2
Clonality
Polyclonal
Conjugation
HRP
Concentration
1 mg/mL
Dilution
ELISA: 1:5,000 - 1:20,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
Purity
Carbonic anhydrase II is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes]. Cross reactivity against Carbonic Anhydrase II from other tissues and species may occur but have not been specifically determined.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Carbonic anhydrase II Peroxidase Conjugated Antibody has been tested by ELISA and dot blot and is assayed against 1.0 ug of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product.
Tested Applications
DOT, ELISA, IHC, WB
NCBI Accession Number
NP_848667.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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