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ARG1 Antibody (HRP)

CAT: 0013-GTR18183954Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18183954Size:25 µL
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Description
Arginase antibody (Peroxidase)
Product Name Alternative
Rabbit anti-Arginase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Arginase Antibody, A I antibody, Al antibody, ARG 1 antibody, ARG1 antibody, Arginase 1 antibody, Arginase liver antibody, Arginase type I antibody, Arginase1 antibody, Liver type arginase antibody, Type I arginase antibody
UniProt
Q2KJ64
Reactivity
Bovine
Immunogen
Arginase [Bovine Liver]
Target
ARG1
Clonality
Polyclonal
Conjugation
HRP
Concentration
1 mg/ml
Dilution
ELISA: 1:10,000 - 1:40,000, WB: 1:1,000 - 1:5,000
Purity
Arginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Arginase [Bovine Liver]. Cross reactivity against Arginase from other tissues and species may occur but have not been specifically determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Arginase Peroxidase Antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Arginase [Bovine Liver] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Tested Applications
DOT, ELISA, WB
NCBI Accession Number
NP_001039619.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · Q2KJ64

Arginase-1

ARGI1_BOVIN · Bos taurus

View on UniProt ↗
Primary accession
Q2KJ64
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ARG1
Protein existence
2: Evidence at transcript level
Organism
Bos taurus (Bovine)
Taxonomy ID
9913
Alternative names
—
EC number
3.5.3.1
Processing
—
Secondary accessions
—
Protein keywords

Biological process

Arginine metabolismUrea cycle

Cellular component

Cytoplasm

Molecular function

Hydrolase

Ligand

ManganeseMetal-binding

PTM

Phosphoprotein

Technical term

Reference proteome

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