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UREA_CANEN Antibody

CAT: 0013-GTR18183917Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18183917Size:25 µL
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Description
Urease antibody
Product Name Alternative
Rabbit anti-Urease Antibody, Urea Amidohydrolase antibody
UniProt
P07374
Reactivity
Plant
Immunogen
Urease [Jack Bean]
Target
UREA_CANEN
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/ml
Dilution
ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
Purity
Anti-Urease is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean]. Cross reactivity against Urease from other tissues and species may occur but have not been specifically determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Urease Antibody has been tested in western blot and suitable against 1.0 ug of Urease [Jack Bean] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product.
Tested Applications
ELISA, IP, WB
NCBI Accession Number
AAA83831.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P07374

Urease

UREA_CANEN · Canavalia ensiformis

View on UniProt ↗
Primary accession
P07374
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
—
Protein existence
1: Evidence at protein level
Organism
Canavalia ensiformis (Jack bean)
Taxonomy ID
3823
Alternative names
—
EC number
3.5.1.5
Processing
—
Secondary accessions
—
Protein keywords

Technical term

3D-structureDirect protein sequencing

Molecular function

HydrolaseNeurotoxinToxin

Ligand

Metal-bindingNickel

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