UREA_CANEN Antibody

CAT: 0013-GTR18183916Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18183916Size:100 µg

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Description

Urease antibody

Product Name Alternative

Rabbit anti-Urease Antibody, Urea Amidohydrolase antibody

UniProt

P07374

Reactivity

Plant

Immunogen

Urease [Jack Bean]

Target

UREA_CANEN

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.0 mg/ml

Dilution

ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000

Purity

Anti-Urease is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean]. Cross reactivity against Urease from other tissues and species may occur but have not been specifically determined.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Urease Antibody has been tested in western blot and suitable against 1.0 ug of Urease [Jack Bean] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product.

Tested Applications

ELISA, IP, WB

NCBI Accession Number

AAA83831.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · P07374

Urease

UREA_CANEN · Canavalia ensiformis

View on UniProt ↗

Primary accession: P07374
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: —
Protein existence: 1: Evidence at protein level
Organism: Canavalia ensiformis (Jack bean)
Taxonomy ID: 3823
Alternative names: —
EC number: 3.5.1.5
Processing: —
Secondary accessions: —

Protein keywords

Technical term

3D-structureDirect protein sequencing

Molecular function

HydrolaseNeurotoxinToxin

Ligand

Metal-bindingNickel

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