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DNASE1, DNL1 Antibody

CAT: 0013-GTR18183899Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18183899Size:25 µL
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Description
Deoxyribonuclease I antibody
Product Name Alternative
Rabbit anti-Deoxyribonuclease I Antibody, Deoxyribonuclease-1, Deoxyribonuclease I, DNase I
UniProt
P00639
Reactivity
Bovine
Immunogen
Deoxyribonuclease A [Bovine Pancreas]
Target
DNASE1, DNL1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Concentration
1 mg/ml
Dilution
ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
Purity
Anti-Deoxyribonuclease I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Deoxyribonuclease A [Bovine Pancreas]. Cross reactivity against Deoxyribonuclease A from other tissues and species may occur but have not been specifically determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Deoxyribonuclease I has been tested by western blot and is suitable to be assayed against 1.0 ug of Deoxyribonuclease A [Bovine Pancreas] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:1,500 of the reconstitution concentration is suggested for this product.
Tested Applications
ELISA, IP, WB
NCBI Accession Number
NP_776959.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P00639

Deoxyribonuclease-1

DNAS1_BOVIN · Bos taurus

View on UniProt ↗
Primary accession
P00639
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
DNASE1
Protein existence
1: Evidence at protein level
Organism
Bos taurus (Bovine)
Taxonomy ID
9913
Alternative names
—
EC number
3.1.21.1
Processing
Precursor
Secondary accessions
A5PK44, Q8MJ27
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Molecular function

Actin-bindingEndonucleaseHydrolaseNuclease

Biological process

Apoptosis

Ligand

Calcium

Cellular component

Cytoplasmic vesicleNucleusSecreted

PTM

Disulfide bondGlycoprotein

Domain

Signal

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