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TRBOTR Antibody (Biotin)

CAT: 0013-GTR18183848Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18183848Size:25 µL
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Description
Trypsin antibody (Biotin)
Product Name Alternative
Rabbit anti-trypsin antibody biotin conjugation, biotin conjugated rabbit anti-trypsin antibody, Anionic trypsinogen antibody, Beta trypsin antibody, Brain trypsinogen antibody, Cationic Trypsinogen antibody, Digestive Zymogen antibody, Mesotrypsin antibody, Mesotrypsinogen antibody
UniProt
P00760
Reactivity
Bovine
Immunogen
Anti-Trypsin Antibody was produced by repeated immunizations with bovine pancreatic trypsin protein purified.
Target
TRBOTR
Clonality
Polyclonal
Conjugation
Biotin
Concentration
1.0 mg/mL
Dilution
ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
Purity
Trypsin Antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsin [Bovine Pancreas]. Cross reactivity against Trypsin from other sources may occur but has not been specifically determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Trypsin Biotin Antibody has been tested by ELISA and western blot. Researchers should determine optimal titers for applications that are not stated below.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
CAA38513.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P00760

Serine protease 1

TRY1_BOVIN · Bos taurus

View on UniProt ↗
Primary accession
P00760
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PRSS1
Protein existence
1: Evidence at protein level
Organism
Bos taurus (Bovine)
Taxonomy ID
9913
Alternative names
—
EC number
3.4.21.4
Processing
Precursor
Secondary accessions
A6H6Y3
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Ligand

CalciumMetal-binding

Biological process

Digestion

PTM

Disulfide bondZymogen

Molecular function

HydrolaseProteaseSerine protease

Cellular component

Secreted

Domain

Signal

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