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PRC1 Antibody (Biotin)

CAT: 0013-GTR18183839Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18183839Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Carboxypeptidase Y antibody (Biotin)
Product Name Alternative
Rabbit anti-Carboxypeptidase Y antibody biotin conjugation, biotin conjugated rabbit anti-Carboxypeptidase Y Antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody, Carboxypeptidase C
UniProt
P00729
Reactivity
Yeast
Immunogen
Carboxypeptidase Y [Baker’s Yeast]
Target
PRC1
Clonality
Polyclonal
Conjugation
Biotin
Concentration
1.0 mg/mL
Dilution
ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
Purity
Anti-Carboxypeptidase Y is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase Y [Baker’s Yeast]. Cross reactivity against Carboxypeptidase Y from other sources may occur but has not been specifically determined.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Carboxypeptidase Y Biotin Antibody has been tested by ELISA and Western Blot and is assayed against 1.0 ug of Carboxypeptidase Y in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent) . Reconstitution Volume: 100 μL
Tested Applications
ELISA, WB
NCBI Accession Number
NP_014026.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P00729

Carboxypeptidase Y

CBPY_YEAST · Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

View on UniProt ↗
Primary accession
P00729
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PRC1
Protein existence
1: Evidence at protein level
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomy ID
559292
Alternative names
CPD-Y, cpY
EC number
3.4.16.5
Processing
Precursor
Secondary accessions
D6W0C4
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Molecular function

CarboxypeptidaseHydrolaseProtease

PTM

Disulfide bondGlycoproteinZymogen

Domain

Signal

Cellular component

Vacuole

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