Products for Research Use Only
RegisterLogin

HIST2H3C Antibody

CAT: 0013-GTR18183794Size: 25 µLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18183794Size:25 µL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Histone H3 K4me1 antibody
Product Name Alternative
Rabbit anti-Anti-Histone H3 Monomethyl Lys4 antibody, rabbit anti-Histone H3 K4 me1 antibody, H3 K4me1, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782
UniProt
Q71DI3
Reactivity
Human
Immunogen
Histone H3 K4me1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 4 of human Histone H3.2.
Target
HIST2H3C
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Concentration
1.0 mg/mL
Dilution
ELISA: 1:3,000 - 1:10,000, ChIP: 2-5μg/million cells, IHC: 2 mg/ml - 5 μg/ml, IF: 2 - 5 μg/ml, WB: 1:500 - 1:2,000
Purity
Anti-Histone H3 [Monomethyl Lys4] was purified from monospecific antiserum by protein A affinity purification. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Histone H3 [Monomethyl Lys4] antibody has been tested in ELISA, Dot blot, and Western Blot. Histone3 K4me1 is useful for Western Blot, Immunocytochemistry, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in the appropriate cell lysate or extract.
Tested Applications
ChIP, DOT, ELISA, IF, IHC, WB
NCBI Accession Number
NP_001005464.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

Related Products

CatalogName
GTR18146305HIST2H3C (27Ac) Antibody
GTR18146306HIST2H3C (27Ac) Antibody
GTR19332512-01HIST2H3C siRNA (Human)
GTR18715102-01HIST2H3C (27Ac) Mouse mAb Antibody
GTR17928534-01HIST1H3A/HIST2H3A/H3F3A Antibody
GTR17928179-01HIST1H3A/HIST2H3A/H3F3A Antibody
GTR17928157-01HIST1H3A/HIST2H3A/H3F3A Antibody
GTR17927917-01HIST1H3A/HIST2H3A/H3F3A Antibody
GTR17927574-01HIST1H3A/HIST2H3A/H3F3A Antibody
GTR17927570-01HIST1H3A/HIST2H3A/H3F3A Antibody

Alternative Products

CatalogName
OACD04205-100ULHIST2H3C Antibody
abx016192HIST2H3C (27Ac) Antibody
BT-MCA2119-01HIST2H3C (27Ac) Monoclonal Antibody
sAP-1238Mouse Monoclonal Antibody to HIST2H3C(27Ac)
RHJ11903Anti-HIST2H3C (27Ac) Antibody (R3V16)
MBS8528371-01Mouse Monoclonal Antibody to HIST2H3C (27Ac)
RHJ11902Anti-HIST2H3C (27Ac) Antibody (R3V15)
AMM81577-01HIST2H3C (27Ac) Mouse Monoclonal Antibody
abx224219Histone H3.2 (HIST2H3C (27Ac) ) Antibody
23396051HIST2H3C Adenovirus (Human)

Popular Products