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BmpA, BB_0383 Antibody

CAT: 0013-GTR18183763Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18183763Size:25 µL
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Description
P39 antibody
Product Name Alternative
Rabbit anti-p39 Antibody, Basic membrane protein A, Borrelia burgdorferi bmpA, immunodominant antigen P39, membrane lipoprotein BmpA
UniProt
Q45010
Reactivity
Bacteria
Immunogen
MBP-fusion protein corresponding to Borrelia burgdorferi p39 protein.
Target
BmpA, BB_0383
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.7 mg/mL
Dilution
WB: 1:1,000
Purity
This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi p39 protein. A BLAST analysis was used to suggest cross-reactivity with p39 from B. burgdorferi and B. afzelii sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against B. garinii sources based on 88-95% homology and to B.spielmanii A14S based on 86-96% homology. Cross-reactivity with p39 from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 35.4 kDa in size corresponding to Borrelia burgdorferi p39 protein by Western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
WP_002656850.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · Q45010

Basic membrane protein A

BMPA_BORBU · Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)

View on UniProt ↗
Primary accession
Q45010
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
bmpA
Protein existence
3: Inferred from homology
Organism
Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31) (Borrelia burgdorferi)
Taxonomy ID
224326
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
P94249, Q44857
Protein keywords

Cellular component

Cell inner membraneCell membraneMembrane

PTM

LipoproteinPalmitate

Technical term

Reference proteome

Domain

Signal

Biological process

Transport

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