Products for Research Use Only
RegisterLogin

IL21 Antibody

CAT: 0013-GTR18183732Size: 25 µLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18183732Size:25 µL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
IL21 antibody
Product Name Alternative
Rabbit anti-IL21 Antibody, Interleukin-21, IL-21, Interleukin21, Interleukin 21, IL 21
UniProt
Q76LU5
Reactivity
Bovine, Equine, Porcine
Immunogen
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to amino acid residues 24-152 of bovine IL-21 protein.
Target
IL21
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Concentration
1.0 mg/mL
Dilution
ELISA: 1:6,000, WB: 1 μg/mL
Purity
This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for bovine IL-21 protein. A BLAST analysis was used to suggest cross-reactivity with IL-21 from bovine sources based on 100% homology with the immunizing sequence. Based on 92% homology, there is a chance of cross-reactivity to porcine IL- 21, 91% to horse IL-21, 89% to human and dog, 88% to macaque, 84% to platypus and Syrian hamster, 76-79% to mouse, 75% to rat, and 48% to chicken. Cross-reactivity with IL-21 from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
IL-21 is expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes, and in HL-60 and THP-1 cell lines. This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15.1 kDa in size corresponding to bovine IL-21 protein by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
Q76LU5.2
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

Related Products

CatalogName
GTR18796687-01IL21 Biosimilar Antibody
GTR18399407-01IL21 polyclonal antibody
GTR18406172-01IL21 Biosimilar Antibody
GTR18796688-01IL21 Biosimilar Antibody
GTR18087155-01IL21 polyclonal antibody
GTR18066860IL21 Polyclonal Antibody
GTR18777468-01IL21 Polyclonal Antibody
GTR18154169-01IL21 Biosimilar Antibody
GTR18695927-01IL21 Human Monoclonal Antibody
GTR18689232-01IL21 Mouse Monoclonal Antibody

Alternative Products

CatalogName
70R-IR062IL21 antibody

Popular Products