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GPC1 Antibody

CAT: 0013-GTR18183728Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18183728Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Glypican-1 antibody
Product Name Alternative
Rabbit anti-Glypican-1 Antibody, Glypican 1, Glypican1, Secreted glypican-1, GPC1, FLJ38078
UniProt
P35052
Reactivity
Human, Mouse, Rat
Immunogen
Anti-Glypican-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human glypican-1 protein.
Target
GPC1
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
2.1 mg/mL
Dilution
ELISA: 1:10,000 - 1:50,000, IHC: 10μg/mL, WB: 1:500- 1:2,000
Purity
Anti-Glypican-1 was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. This antibody is specific for human glypican-1 protein. A BLAST analysis was used to suggest partial cross-reactivity with glypican from rat, mouse, Macaque, dog, cattle, and opossum sources based on 100 - 88% homology with the immunizing sequence. Cross-reactivity with glypican from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Glypican-1 protein A purified antibody has been tested for use in ELISA, and immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~61 kDa in size corresponding to glypican by western blotting in the appropriate cell lysate or extract. The higher molecular weight (110kDa) of transfected Fc-glypican compared with the expected MW of glypican is likely due to the presence of the Fc-tag.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
30410985
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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