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SGK1 Antibody

CAT: 0013-GTR18183726Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18183726Size:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SKG-1 antibody
Product Name Alternative
Rabbit anti-SKG-1 Antibody, Serum/glucocorticoid-regulated kinase 1, SGK 1 antibody, SGK1 antibody, Cellular stress response antibodies, Serine/threonine-protein kinase Sgk1
UniProt
O00141
Reactivity
Human
Immunogen
SKG-1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 400-431 of human SGK-1.
Target
SGK1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Concentration
5.0 mg/mL
Dilution
ELISA: 1:4,000 - 1:16,000, IHC: 10 μg/ml to 20 μg/ml, WB: 1:500 - 1:3,000
Purity
Anti-SKG-1 Antibody is directed against human SGK-1 protein. The product was protein A purified from monospecific antiserum. A BLAST analysis was used to suggest reactivity with this protein from human, macaque and chimpanzee sources based on 100% homology for the immunogen sequence. This antibody is expected to cross react with SGK-1 homologues from mouse, rat, dog, chicken, bovine, rabbit and spiny dogfish based on an 92% sequence homology for the immunogen (12/13) from these sources. Cross reactivity with SGK-1 homologues from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store SKG-1 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
SKG-1 Antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~49 kDa in size corresponding to SGK-1 by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
O00141.2
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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