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B2M Antibody

CAT: 0013-GTR18183711Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18183711Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Beta-2-Microglobulin antibody
Product Name Alternative
Rabbit anti-Beta-2-Microglobulin Antibody, b2-Microglobulin, B2M antibody, B2MG_HUMAN antibody, Beta 2 microglobin antibody, Beta 2 microglobulin precursor antibody, Beta chain of mhc class 1 proteins antibody, Hdcma22p antibody, CDABP0092 antibody, IMD43 antibody
UniProt
P61769
Reactivity
Human
Immunogen
Beta-2-Microglobulin [Human Urine]
Target
B2M
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Concentration
1.0 mg/ml
Dilution
ELISA: 1:2,000 - 1:10,000, IHC: 1:500 - 1:2,000, IF: 1:200, WB: 1:1,000 - 1:5,000
Purity
Anti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified b2-Microglobulin [Human Urine]. Cross reactivity against b2-Microglobulin from other sources is unknown.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-beta-2-Microglobulin antibody has been tested in western blot, immunohistochemistry, and immunofluorescence. This product detects a single band of the expected apparent molecular weight (~13.7kDa), and is suitable for use in ELISA. Researchers should determine optimal titers for applications that are not stated below.
Tested Applications
ELISA, IF, IHC, WB
NCBI Accession Number
NP_004039.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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