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AKT1 Antibody

CAT: 0013-GTR18183648Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18183648Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
AKT1 antibody
Product Name Alternative
Mouse anti-AKT1 antibody, AKT-1, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 1 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma
UniProt
P31749
Reactivity
Human, Mouse
Immunogen
Anti-AKT1 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein.
Target
AKT1
Clonality
Monoclonal
Clone
14E5.A2.B2.H9
Conjugation
Unconjugated
Field of Research
Signal Transduction
Concentration
1.0 mg/ml
Dilution
ELISA: 1:2,000 - 1:10,000, IHC: 20 μg/mL, WB: 1:1000
Purity
Anti-AKT1 antibody is directed against human AKT1. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-AKT1 Antibody has been tested in ELISA, SDS-Page, flow cytometry, and western blotting. This antibody is suitable for immunoprecipitation and immunohistochemistry. Expect a band approximately 56 kDa in size corresponding to AKT1 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
Tested Applications
ELISA, FC, IHC, WB
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG2a

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