TRPC6 Antibody

CAT: 0013-GTR18183585Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18183585Size:100 µg

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Description

TRPC6 antibody

Product Name Alternative

Mouse anti-TRPC6 Antibody, TRPC 6, TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6

UniProt

Q9Y210

Reactivity

Human, Mouse

Immunogen

This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human TRPC6 protein.

Target

TRPC6

Clonality

Monoclonal

Clone

3F2.H10.F2

Conjugation

Unconjugated

Field of Research

Neuroscience

Concentration

0.964 mg/mL

Dilution

ELISA: 1:10,000 - 1:50,000, IHC: 2.5 μg/mL, WB: 1:500- 1:2,000

Purity

This product was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human TRPC6 protein. A BLAST analysis was used to suggest cross-reactivity with TRPC6 from chimpanzee based on 100% homology with the immunizing sequence. Cross-reactivity with TRPC6 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-TRPC6 monoclonal antibody has been tested by ELISA, immunohistochemistry and western blotting. Expect a band approximately 30 kDa in size corresponding to the cytoplasmic domain of TRPC6 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required. Strong staining was observed in adrenal, Purkinje neurons, cortical neurons, heart, ganglion cells, renal tubules, Sertoli cells, hepatocytes, skeletal muscle, exocrine pancreas, and germinal centers of lymphoid follicles. Moderate staining was observed in colon epithelium and plasma cells, B-lymphocytes, and parafollicular cells of the thyroid. Faint staining was seen in respiratory epithelium. Prostate and placenta were negative for staining. The antibody produced minimal to no background staining and appeared very specific at 2.5 μg/mL.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

5730102

Host or Source

Mouse

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG1

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