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AKT1 Antibody

CAT: 0013-GTR18183564Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18183564Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Akt (phospho-S473) antibody
Product Name Alternative
Mouse anti-AKT pS473 Antibody, phospho AKT, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1
UniProt
P31749
Reactivity
Human, Monkey, Mouse, Rat
Immunogen
Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein, followed by hybridoma development.
Target
AKT1
Clonality
Monoclonal
Clone
17F6.B11
Conjugation
Unconjugated
Field of Research
Signal Transduction
Concentration
1.0 mg/ml
Dilution
ELISA: 1:20,000, IHC: 20 μg/mL, IF: 1:500-1:3,000, WB: 1:500-1:3,000
Purity
Anti-AKT pS473 Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This phospho specific monoclonal antibody is specific for phosphorylated human and mouse AKT protein at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined.
Form
Liquid (sterile filtered)
References & Citations
Deng, Dan et al. Silencing MEG3 protects PC12 cells from hypoxic injury by targeting miR-21 Artif Cells Nanomed Biotechnol, 48, 610-619 (2020), Wang, Weiwei et al. Notoginsenoside R1 alleviates high glucose-evoked damage in RSC96 cells through down-regulation of miR-503 Artif Cells Nanomed Biotechnol, 47, 3947-3954 (2019), Chen, Yanbo et al. Pretreatment of ghrelin protects H9c2 cells against hypoxia/reoxygenation-induced cell death via PI3K/AKT and AMPK pathways Artif Cells Nanomed Biotechnol, 47, 2179-2187 (2019), Li, Meng et al. Melatonin Inhibits the Ferroptosis Pathway in Rat Bone Marrow Mesenchymal Stem Cells by Activating the PI3K/AKT/mTOR Signaling Axis to Attenuate Steroid-Induced Osteoporosis Oxid Med Cell Longev, 2022, 8223737 (2022), Shouyu Xiang et al. miR-19b-3p regulates autophagy of BMSCs in hypoxia through PTEN/Akt/mTOR pathway to promote fracture healing J Mol Histol, 57 (1), 30 (2026)
Pubmed id
32050796, 31581849, 31159591, 36035224, 41495593
Storage Conditions
Store Anti-AKT pS473 (MOUSE) Monoclonal Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Phospho AKT antibody is tested in ELISA, immunofluorescence, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required.
Tested Applications
ELISA, IF, IHC, Multiplex Assay, WB
NCBI Accession Number
62241011
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG1

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