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RELA Antibody

CAT: 0013-GTR18183552Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18183552Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NFkB p65 antibody
Product Name Alternative
Mouse anti-NF-kB p65 Antibody, mouse anti-Rel A antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB, Nuclear factor NF-kappa-B p65 subunit
UniProt
Q04206
Reactivity
Human
Immunogen
NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) .
Target
RELA
Clonality
Monoclonal
Clone
27F9.G4
Conjugation
Unconjugated
Concentration
0.98 mg/mL
Dilution
ELISA: 1:50,000-1:100,000, IHC: 1:200-1:600, IF: 1:5,000, WB: 1:1,000-1:5,000
Purity
Anti-NF-kB p65 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography and showed a single band by IEP (immunoelectrophoresis) when tested with anti-mouse antibody. Reactivity was confirmed by ELISA against peptide conjugated carrier protein and by Western blot against HeLa whole cell lysate.
Form
Liquid (sterile filtered)
References & Citations
Deng, Dan et al. Silencing MEG3 protects PC12 cells from hypoxic injury by targeting miR-21 Artif Cells Nanomed Biotechnol, 48, 610-619 (2020), Yang, Xifeng et al. Downregulation of miR‐181b inhibits human colon cancer cell proliferation by targeting CYLD and inhibiting the NF‐κB signaling pathway Int J Mol Med, 46, 1755-1764 (2020), Li, Ying et al. Pterostilbene pre-treatment reduces LPS-induced acute lung injury through activating NR4A1 Pharm Biol, 60, 394-403 (2022)
Pubmed id
32050796, 32901872, 35271397
Storage Conditions
Store NF-kB p65 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-NF-kB p65 Antibody is a mouse monoclonal antibody directed against NFkB p65 (Rel A) and recognizes a 65 kD band by Western blot against HeLa whole cell lysate. Control peptide is sold separately. This product tested in WB, ICC, IHC, and IF
Tested Applications
ELISA, IF, IHC, WB
NCBI Accession Number
223468676
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG2a

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