ALDOA Antibody (HRP)

CAT: 0013-GTR18184190Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18184190Size:100 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Aldolase antibody (Peroxidase)

Product Name Alternative

Goat anti-Aldolase Antibody, HRP conjugated goat anti-Aldolase Antibody, Fructose-bisphosphate aldolase A, Muscle-type aldolase, peroxidase Aldolase

UniProt

P00883

Reactivity

Human, Rabbit

Immunogen

Aldolase [Rabbit Muscle]

Target

ALDOA

Clonality

Polyclonal

Conjugation

HRP

Field of Research

Metabolism Research

Concentration

1.0 mg/mL

Dilution

ELISA: 1:1,000 - 1:5,000, IP: 1:100, WB: 1:500 - 1:5,000

Purity

Aldolase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Aldolase [Rabbit Muscle].

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Aldolase has been tested by ELISA, immunoprecipitation, and western blot. This product is assayed against 1.0 μg of Aldolase [Rabbit Muscle] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product.

Tested Applications

ELISA, IP, WB

NCBI Accession Number

NP_001075707.1

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · P00883

Fructose-bisphosphate aldolase A

ALDOA_RABIT · Oryctolagus cuniculus

View on UniProt ↗

Primary accession: P00883
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: ALDOA
Protein existence: 1: Evidence at protein level
Organism: Oryctolagus cuniculus (Rabbit)
Taxonomy ID: 9986
Alternative names: —
EC number: 4.1.2.13
Processing: —
Secondary accessions: Q28671

Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

PTM

AcetylationHydroxylationIsopeptide bondPhosphoproteinUbl conjugation

Cellular component

Cytoplasm

Biological process

Glycolysis

Molecular function

Lyase

Ligand

Schiff base

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