ChoA Antibody (Biotin)

CAT: 0013-GTR18183658Size: 25 µLDry Ice: NoHazardous: No

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CAT#:0013-GTR18183658Size:25 µL

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Description

Cholesterol Oxidase antibody (Biotin)

Product Name Alternative

Goat anti-Cholesterol Oxidase Antibody biotin Conjugation, biotin Conjugated goat anti-Cholesterol Oxidase Antibody, choA antibody, choD antibody, choM antibody, choS antibody, COD antibody

UniProt

P12676

Reactivity

Bacteria

Immunogen

Cholesterol Oxidase [Streptomyces]

Target

ChoA

Clonality

Polyclonal

Conjugation

Biotin

Concentration

1 mg/ml

Dilution

ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000

Purity

Anti-Cholesterol Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Cholesterol Oxidase [Microorganism]. Cross reactivity against Cholesterol Oxidase from other sources is unknown.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Cholesterol Oxidase has been tested by ELISA, dot blot, and western blot. This product is assayed against 1.0 ug of Cholesterol Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product

Tested Applications

DOT, ELISA, IP, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · P12676

Cholesterol oxidase

CHOD_STRS0 · Streptomyces sp. (strain SA-COO)

View on UniProt ↗

Primary accession: P12676
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: choA
Protein existence: 1: Evidence at protein level
Organism: Streptomyces sp. (strain SA-COO)
Taxonomy ID: 74576
Alternative names: CHOD
EC number: 1.1.3.6
Processing: Precursor
Secondary accessions: —

Protein keywords

Technical term

3D-structureDirect protein sequencing

Biological process

Cholesterol metabolismLipid metabolismSteroid metabolismSterol metabolism

Ligand

FADFlavoprotein

Molecular function

IsomeraseOxidoreductase

Cellular component

Secreted

Domain

Signal

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