Products for Research Use Only

GPD1 Antibody (Biotin)

CAT: 0013-GTR18183580Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18183580Size:100 µg
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Description
Glycerol-3-Phosphate Dehydrogenase antibody (Biotin)
Product Name Alternative
Goat anti-Glycerol-3-Phosphate Dehydrogenase Antibody biotin Conjugation, biotin Conjugated goat anti-Glycerol-3-Phosphate Dehydrogenase Antibody, FLJ26652 antibody, G3PD antibody, Gdc-1 antibody, Glycerphosphate dehydrogenase antibody, GPD-C antibody, Gpd1 protein antibody
UniProt
P08507
Reactivity
Rabbit
Immunogen
Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle]
Target
GPD1
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Metabolism Research
Concentration
1.0 mg/mL
Dilution
ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,500
Purity
Anti-Glycerol-3-Phosphate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle]. Cross reactivity against Glycerol-3-Phosphate-Dehydrogenase from other sources is unknown.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Glycerol-3-Phosphate Dehydrogenase has been tested by ELISA and is assayed against 1.0 ug of Glycerol-3-Phosphate-Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent) . Reconstitution Volume: 100 μL
Tested Applications
ELISA, WB
NCBI Accession Number
3043365
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P08507

Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic

GPDA_RABIT · Oryctolagus cuniculus

View on UniProt ↗
Primary accession
P08507
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
GPD1
Protein existence
3: Inferred from homology
Organism
Oryctolagus cuniculus (Rabbit)
Taxonomy ID
9986
Alternative names
GPD-C, GPDH-C
EC number
1.1.1.8
Processing
—
Secondary accessions
—
Protein keywords

Cellular component

Cytoplasm

Ligand

NAD

Molecular function

Oxidoreductase

PTM

Phosphoprotein

Technical term

Reference proteome