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GST Antibody

CAT: 0013-GTR18183475Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18183475Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
GST antibody
Product Name Alternative
Goat anti-GST antibody, GST, Glutathione-S-Transferase
Immunogen
Glutathione-S-Transferase [Schistosoma japonicum]
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/ml
Dilution
ELISA: 1:200 - 1:1000, WB: 1:1000 - 1:5000
Purity
Anti-GST is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store GST antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-GST antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Glutathione-S-Transferase [Schistosoma japonicum] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 of the reconstitution concentration is suggested for this product. Specific conditions for reactivity should be optimized by the end user.
Tested Applications
ELISA, WB
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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