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YcjM Antibody

CAT: 0013-GTR18183459Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18183459Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Sucrose phosphorylase antibody
Product Name Alternative
Goat anti-Sucrose Phosphorylase Antibody, Putative sucrose phosphorylase, Sucrose glucosyltransferase
UniProt
P76041
Reactivity
E. coli
Immunogen
Sucrose Phosphorylase [E.coli]
Target
YcjM
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1 mg/mL
Dilution
ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
Purity
Anti-Sucrose phosphorylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Sucrose Phosphorylase [E.coli]. Cross reactivity against Sucrose Phosphorylase from other sources is unknown.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Sucrose phosphorylase Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Sucrose Phosphorylase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:6,500 to 1:32,000 of the reconstitution concentration is suggested for this product.
Tested Applications
ELISA, IP, WB
NCBI Accession Number
YP_852475.1
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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