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GPD1 Antibody

CAT: 0013-GTR18183445Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18183445Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Glycerol-3-Phosphate Dehydrogenase antibody
Product Name Alternative
Goat anti-Glycerol-3-Phosphate Dehydrogenase Antibody, FLJ26652 antibody, G3PD antibody, Gdc-1 antibody, Glycerphosphate dehydrogenase antibody, GPD-C antibody, Gpd1 protein antibody
UniProt
P08507
Reactivity
Rabbit
Immunogen
Glycerol-3-Phosphate Dehydrogenase [Rabbit Muscle]
Target
GPD1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Metabolism Research
Concentration
1.0 mg/ml
Dilution
ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
Purity
Anti-GLYCEROL-3-PHOSPHATE DEHYDROGENASE (GOAT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glycerol-3-Phosphate Dehydrogenase [Rabbit Muscle]. Cross reactivity against Glycerol-3-Phosphate Dehydrogenase from other sources is unknown.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-GLYCEROL-3-PHOSPHATE DEHYDROGENASE (GOAT) Antibody has been assayed against 1.0 ug of Glycerol-3-Phosphate Dehydrogenase [Rabbit Muscle] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Specific conditions should be optimized by researcher.
Tested Applications
ELISA, WB
NCBI Accession Number
3043365
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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