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ABC3735 Antibody

CAT: 0013-GTR18183426Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18183426Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Uricase antibody
Product Name Alternative
Goat anti-uricase antibody, Urate oxidase antibody, Factor-independent urate hydroxylase
UniProt
Q5WBJ3
Reactivity
Bacteria
Immunogen
Uricase [Bacillus species]
Target
ABC3735
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/ml
Dilution
ELISA: 1:1,000 - 1:2,500, IP: 1:100, WB: 1:500 - 1:1,000
Purity
Anti-Uricase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Uricase [Bacillus species]. Cross reactivity against Uricase from other sources is unknown.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Uricase Antibody has been tested by western blot and is assayed against 1.0 ug of Uricase [Bacillus species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:3,000 of the reconstitution concentration is suggested for this product.
Tested Applications
ELISA, IP, WB
NCBI Accession Number
YP_177228.1
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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