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Protein A Antibody

CAT: 0013-GTR18183425Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18183425Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Protein A antibody
Product Name Alternative
Goat anti-Protein A Antibody, goat anti Protein A, goat anti PA
Immunogen
Protein A [Staphylococcus aureus]
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/mL
Dilution
ELISA: 1:20,000 - 1:100,000, IP: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Purity
Protein A Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A [Staphylococcus aureus] and anti-Goat Serum. Cross reactivity against Protein A from other sources is unknown.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Protein A Antibody has been tested by western blot and is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity. Optimal concentration in immunoassays should be determined by the researcher.
Tested Applications
ELISA, IP, WB
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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