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GLUD1 Antibody (HRP)

CAT: 0013-GTR18183974Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18183974Size:25 µL
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Description
Glutamate Dehydrogenase antibody (Peroxidase)
Product Name Alternative
Rabbit anti-Glutamate Dehydrogenase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Glutamate Dehydrogenase Antibody, Glutamate dehydrogenase 1 mitochondrial, GDH 1
UniProt
P00366
Reactivity
Bovine
Immunogen
Glutamate Dehydrogenase [Bovine Liver]
Target
GLUD1
Clonality
Polyclonal
Conjugation
HRP
Field of Research
Metabolism Research
Concentration
1.0 mg/mL
Dilution
IP: 1:100, WB: 1:250
Purity
Glutamate dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Glutamate Dehydrogenase [Bovine Liver]. Cross reactivity against Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Glutamate dehydrogenase has been assayed against 1.0 ug of Glutamate Dehydrogenase [Bovine Liver] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Tested Applications
IP, WB
NCBI Accession Number
32880221
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P00366

Glutamate dehydrogenase 1, mitochondrial

DHE3_BOVIN · Bos taurus

View on UniProt ↗
Primary accession
P00366
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
GLUD1
Protein existence
1: Evidence at protein level
Organism
Bos taurus (Bovine)
Taxonomy ID
9913
Alternative names
GDH 1
EC number
1.4.1.3
Processing
Precursor
Secondary accessions
Q3SYY0, Q7YS29, Q8HZ49
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

PTM

AcetylationADP-ribosylationHydroxylationPhosphoprotein

Ligand

ATP-bindingGTP-bindingNADPNucleotide-binding

Cellular component

Endoplasmic reticulumMitochondrion

Molecular function

Oxidoreductase

Domain

Transit peptide

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