ARG1 Antibody

CAT: 0013-GTR18183876Size: 25 µLDry Ice: NoHazardous: No

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CAT#:0013-GTR18183876Size:25 µL

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Description

Arginase antibody

Product Name Alternative

Rabbit anti-Arginase Antibody, A I antibody, Al antibody, ARG 1 antibody, ARG1 antibody, Arginase 1 antibody, Arginase liver antibody, Arginase type I antibody, Arginase1 antibody, Liver type arginase antibody, Type I arginase antibody

UniProt

Q2KJ64

Reactivity

Bovine

Immunogen

Arginase [Bovine Liver]

Target

ARG1

Clonality

Polyclonal

Conjugation

Unconjugated

Dilution

ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000

Purity

Anti-Arginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Arginase [Bovine Liver]. Cross reactivity against Arginase from other tissues and species may occur but have not been specifically determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Arginase Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Arginase [Bovine Liver] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:500,000 to 1:2,000,000 of the reconstitution concentration is suggested for this product.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_001039619.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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